Proteomic profiling of extracellular vesicles secreted from Toxoplasma gondii

Proteomics. 2017 Jun 23. doi: 10.1002/pmic.201600477. [Epub ahead of print]

Wowk PF1, Zardo ML1, Miot HT1, Goldenberg S1, Carvalho PC1, Mörking PA1.

Author information

Abstract

Toxoplasma gondii infects a wide range of hosts worldwide, including humans and domesticated animals causing toxoplasmosis disease. Recently, exosomes, small extracellular vesicles (EV) that contain nucleic acids, proteins, and lipids derived from their original cells were linked with disease protection. The effect of EVs derived from T. gondii on the immune response and its relevance in a physiological context is unknown. Here we disclose the first proteomic profiling of T. gondii EVs compared to EVs isolated from a human foreskin fibroblast infected cell line cultured in a vesicle- free medium. Our results reveal a broad range of canonical exosomes proteins. Data are available via ProteomeXchange with identifier PXD004895. This article is protected by copyright. All rights reserved.

This article is protected by copyright. All rights reserved.

KEYWORDS: 

Extracellular vesicles; MS; Toxoplasma gondii; Toxoplasma-infected cell

PMID:

 

28643940

 

DOI:

 

10.1002/pmic.201600477