One minute ultraviolet exposure inhibits Toxoplasma gondii tachyzoite replication and cyst conversion without diminishing host humoral-mediated immune response

Exp Parasitol. 2014 Aug 14. pii: S0014-4894(14)00191-X. doi: 10.1016/j.exppara.2014.08.001. [Epub ahead of print

 

Kannan G¹, Prandovszky E², Steinfeldt CB², Gressitt KL², Yang C³, Yolken RH², Severance EG², Jones-Brando L², Pletnikov MV⁴

 

We developed a protocol to inactivate Toxoplasma gondii (T. gondii) tachyzoites employing 1 minute of ultraviolet (UV) exposure. We show that this treatment completely inhibited parasite replication and cyst formation in vitro and in vivo but did not affect the induction of a robust IgG response in mice. We propose that our protocol can be used to study the contribution of the humoral immune response to rodent behavioral alterations following T. gondii infection.
Copyright © 2014. Published by Elsevier Inc.

KEYWORDS:

Animal models; Behavior; Immune response; Inactivation, Cysts; Toxoplasma

PMID:

25131777

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