Cell Rep. 2014 May 21. pii: S2211-1247(14)00333-7. doi: 10.1016/j.celrep.2014.04.031. [Epub ahead of print]

Impact of Regulated Secretion on Antiparasitic CD8 T Cell Responses

Grover HS¹, Chu HH¹, Kelly FD², Yang SJ¹, Reese ML², Blanchard N³, Gonzalez F¹, Chan SW¹, Boothroyd JC², Shastri N⁴, Robey EA⁵.

Abstract

CD8 T cells play a key role in defense against the intracellular parasite Toxoplasma, but why certain CD8 responses are more potent than others is not well understood. Here, we describe a parasite antigen, ROP5, that elicits a CD8 T cell response in genetically susceptible mice. ROP5 is secreted via parasite organelles termed rhoptries that are injected directly into host cells during invasion, whereas the protective, dense-granule antigen GRA6 is constitutively secreted into the parasitophorous vacuole. Transgenic parasites in which the ROP5 antigenic epitope was targeted for secretion through dense granules led to enhanced CD8 T cell responses, whereas targeting the GRA6 epitope to rhoptries led to reduced CD8 responses. CD8 T cell responses to the dense-granule-targeted ROP5 epitope resulted in reduced parasite load in the brain. These data suggest that the mode of secretion affects the efficacy of parasite-specific CD8 T cell responses.
Copyright © 2014 The Authors. Published by Elsevier Inc. All rights reserved.

PMID:

24857659

[PubMed - as supplied by publisher]