Exp Parasitol. 2014 Apr 23. pii: S0014-4894(14)00097-6. doi: 10.1016/j.exppara.2014.04.008. [Epub ahead of print]

Influence of infection by Toxoplasma gondii on purine levels and E-ADA activity in the brain of mice experimentally infected mice

Tonin AA¹, Da Silva AS², Casali EA³, Silveira SS⁴, Moritz CE⁴, Camillo G⁵, Flores MM⁶, Fighera R⁶, Thomé GR⁷, Morsch VM⁷, Schetinger MR⁷, De La Rue M⁸, Vogel FS⁵, Lopes ST⁸.

 

Abstract

The aim of this study was to assess the purine levels and E-ADA activity in the brain of mice (BALB/c) experimentally infected with Toxoplasma gondii. In experiment I (n=24) the mice were infected with RH strain of T. gondii, while in experiment II (n=36) they were infected with strain ME-49 of T. gondii. Our results showed that, for RH strain (acute phase), an increase in both periods in the levels of ATP, ADP, AMP, adenosine, hypoxanthine, xanthine (only on day 6 PI) and uric acid (only on day 6 PI). By the other hand, the RH strain led, on days 4 and 6 PI, to a reduction in the concentration of inosine. ME-49, a cystogenic strain, showed some differences in acute and chronic phase, since on day 6 PI the levels of ATP and ADP were increased, while on day 30 these same nucleotides were reduced. On day 60 PI, ME-49 induced a reduction in the levels of ATP, ADP, AMP, adenosine, inosine and xanthine, while uric acid was increased. A decrease of E-ADA activity was observed in brain on days 4 and 6 PI (RH), and 30 PI (ME-49); however on day 60 PI E-ADA activity was increased for infection by ME-49 strain. Therefore, it was possible to conclude that infection with T. gondii changes the purine levels and the activity of E-ADA in brain, which may be associated with neurological signs commonly observed in this disease.
Copyright © 2014. Published by Elsevier Inc.

KEYWORDS:

Ectoenzymes, ME-49, Purinergic signaling, RH, Toxoplasmosis

PMID:

24768956

[PubMed - as supplied by publisher]