Thursday, June 30, 2011

Toxoplasma gondii peroxiredoxin promotes altered macrophage function, caspase-1-dependent IL-1beta secretion enhances parasite replication

Vet Res. 2011 Jun 27;42(1):80. [Epub ahead of print]

Toxoplasma gondii peroxiredoxin promotes altered macrophage function, caspase-1-dependent IL-1beta secretion enhances parasite replication.

Marshall ES, Elsheikha HM, Hakimi MA, Flynn RJ.

Abstract
ABSTRACT: Alternatively activated macrophages (AAM) are a key feature Th2 immunity and have been associated with a variety of roles during helminth infection. The role this cell subset plays in protzoan infection remain relatively unexplored, herein we describe the effects of a redox enzyme (rTgPrx) derived from Toxoplasma gondii on murine macrophage phenotype in vitro. RTgPrx has been previously associated with the maintainence of parasite oxidative balance. Here our experiments show that rTgPrx promotes AAM as indicated by high arginase-1 (arg-1), YM1 and FIZZ expression via both signal transducer and activator of transcription (STAT)6-dependent and -independent mechanisms. Additionally rTgPrx treatment reduced caspase-1 activity and IL-1beta secretion, while simultaneously increasing IL-10 release. Furthermore the in vitro replication of T. gondii (RH strain) was enhanced when macrophages were treated with rTgPrx. This is in contrast with the previously described effects of a Plasmodium berghei ANKA 2-cys-peroxiredoxin that promotes pro-inflammatory cytokine production. These results highlight the role of T. gondii derived redox enzymes as important immune modulators and potentially indicate a role for AAM in modulating immunopathology and promoting parasite replication during T. gondii infection.

PMID:21707997[PubMed - as supplied by publisher]

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