Electrophoresis. 2010 Mar 23. [Epub ahead of print]
Separation of DNA-containing organelles from Toxoplasma gondii by CZE
Moe MK, Samuelsen PJ, Nielsen HV, Nielsen KM.
Department of Pharmacy, University of Tromsø, Tromsø, Norway.
Toxoplasma gondii and other members of the family Apicomplexa have two organelles, in addition to the nucleus, that contain DNA. Herein is reported the separation of the DNA-carrying organelles from T. gondii tachyzoites, i.e. the mitochondrion and the apicoplast, by CZE. The cells were stained with SYTO9, a dye that exhibit fluorescence when interacting with double stranded nucleic acids (e.g. DNA) and disrupted by nitrogen cavitation. Following careful removal of the heavier cellular material, the remaining lysate was injected on a CE instrument and the DNA-containing organelles were detected by LIF. The mitochondrion had longer migration time than the apicoplast, and the migration times were comparable in the replicates. This method should potentially also work for other members of the Apicomplexa including Plasmodium falciparum.
PMID: 20333721 [PubMed - as supplied by publisher]
4 comments:
It's a magnificent blog!!It's really useful for us!
Xamarin Training in Chennai
Xamarin Course
IoT courses in Chennai
Node JS Course in Chennai
Big Data Analytics Training in Chennai
Hadoop Admin Training in Chennai
informatica mdm training
Nice Blog. the blog is really impressive every content should be very uniquely represented.
Data Science Training Course In Chennai | Data Science Training Course In Anna Nagar | Data Science Training Course In OMR | Data Science Training Course In Porur | Data Science Training Course In Tambaram | Data Science Training Course In Velachery
At the point when you download YouTube videos by our free YouTube Downloader, it doesn't mean you need to watch them on one device. Free Youtube Downloader Serial Key
This priceless threat data give us unbelievable insight into what’s occurrence at the present. This virus can also assault the windows operating scheme that make the records and individual data become tainted. Malwarebytes Anti Malware Premium Keys
Post a Comment