Resonance assignments for Toxo proteins

Biomol NMR Assign. 2009 Jun;3(1). Epub 2009 Feb 27.

Complete resonance assignments for the MIC2 associated protein from Toxoplasma gondii

Liu B, Sawmynaden K, Marchant J, Simpson P, Matthews S.

Division of Molecular Biosciences, Department of Life Sciences, Imperial College London, South Kensington, London, SW7 2AZ, UK.

Toxoplasma gondii is an obligate parasite that infects most warm blood animals. Micronemal proteins actively involves in the invasion process, where TgMIC2 and TgM2AP complex plays vital roles. Complete NMR assignments for major fragment of TgM2AP were successfully obtained.

Publication Types:
Research Support, Non-U.S. Gov't

PMID: 19636952 [PubMed - in process]

Complete NMR assignments for the second EGF domain of MIC6 from Toxoplasma gondii and re-assignment in complex with the galectin-like domain of MIC1

Sawmynaden K, Saouros S, Marchant J, Simpson P, Matthews S.

Division of Molecular Biosciences, Department of Life Sciences, Imperial College London, South Kensington, London, SW7 2AZ, UK.

Toxoplasma gondii is the causative agent of toxoplasmosis. Here we present a complete set of NMR assignments for the second EGF domain from microneme protein 6 and its re-assignment in complex with the galectin-like domain from microneme protein 1.

Publication Types:
Research Support, Non-U.S. Gov't

PMID: 19636901 [PubMed - in process]

Complete resonance assignment of the galectin-like domain of MIC1 from Toxoplasma gondii in complex with the second EGF domain from MIC6 and the backbone assignment in complex with the third EGF domain

Saouros S, Sawmynaden K, Marchant J, Simpson P, Matthews S.

Division of Molecular Biosciences, Imperial College London, South Kensington Campus, London, SW7 2AZ, UK.

Microneme protein complexes are important for invasion of host cells by Toxoplasma gondii. We report the resonance assignment of the galectin-like domain of microneme protein 1 in complexes with the second and third EGF domains from microneme protein 6.

Publication Types:
Research Support, Non-U.S. Gov't

PMID: 19636898 [PubMed - in process]

Complete resonance assignment of the first and second apple domains of MIC4 from Toxoplasma gondii, using a new NMRView-based assignment aid

Marchant J, Sawmynaden K, Saouros S, Simpson P, Matthews S.

Division of Molecular Biosciences, Department of Life Sciences, Imperial College London, South Kensington, London, SW7 2AZ, UK.

Microneme protein 4 is involved in cell binding by the important parasite Toxoplasma gondii. We present here the backbone and side-chain assignments of the first two apple domains together with a new graphical aid for their assignment using NMRView.

Publication Types:
Research Support, Non-U.S. Gov't

PMID: 19636884 [PubMed - in process]