This study evaluated levels for mRNA expression of 7 cytokines in ocular toxoplasmosis. Peripheral blood mononuclear cells (PBMC) of ocular toxoplasmosis patients (OT Group, n= 23) and chronic toxoplasmosis individuals (CHR Group, n= 9) were isolated and stimulated in vitro with T. gondii antigen. Negative controls (NC) were constituted of 7 PBMC samples from individuals seronegative for toxoplasmosis. mRNA expression for cytokines was determined by qPCR. Results showed a significant increase of mRNA levels from antigen stimulated PBMCs derived from OT Group for expressing IL-6 (at p0.005 and p 0.0005 for CHR and NC groups, respectively), IL-10 (at p0.0005 and p 0.005 for CHR and NC groups, respectively) and TGF-β (at p 0.005) for NC group. mRNA levels for TNF-α and IL-12 were also up-regulated in OT patients compared to CHR and NC individuals, although without statistical significance. Additionally, mRNA levels for IL-27 and IFN-γ in PBMC of OT patients were up-regulated in comparison with NC individuals. Differences between OT and NC groups were statistically significant at p0.05 and p 0.0005, respectively. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.
KEYWORDS:
PBMC ; Cytokines; Gene expression; Ocular toxoplasmosis
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