The effects of extremely low frequency electromagnetic fields (ELF-EMF) onToxoplasma gondiihave not been explained yet. The aim of this study was to assess the possible effects of ELF-EMF on growth, survival time and viability ofToxoplasma gondii. In addition, the life span ofToxoplasmainfected animals was investigated.
Sixty adult male BALB/c mice were used for in vivo and in vivo experiments in Laboratory of Biopyhsics and Parasitology of Medical Faculty, Adnan Menderes University, Turkey, in 2010. During in vivo experiments, pulsed and continuous EMFs were applied for 5 d to the infected mice. During in vivo experiments, pulsed and continuous EMF was applied to the tachyzoites within peritoneal exudates for 8 h/d at 4 °C and the tachyzoites were then injected to mice. In both experiments, the number ofT. gondiiin peritoneal exudates was counted andT. gondiiprotein bands patterns were investigated with polyacrylamide gel electrophoresis and Western Blotting.
Pulsed and continuous EMF exposure reduced the number ofT. gondiitachyzoites in comparison to controls. However, no statistically significant differences were observed at the patterns of protein bands among the samples.
EMF exposure induces a decrease in the number ofT. gondii. Further studies are required to understand the mechanism of EMF on intracellular parasites.
Electromagnetic field; Mice; Polyacrylamide gel electrophoresis; Toxoplasma gondii; Western Blotting