Thursday, July 07, 2016

Transnuclear CD8 T cells specific for the immunodominant epitope Gra6 lower acute phase Toxoplasma gondii burden

 2016 Jul 5. doi: 10.1111/imm.12643. [Epub ahead of print]


We generated a CD8 T cell receptor transnuclear (TN) mouse specific to the Ld -restricted immunodominant epitope of GRA6 from Toxoplasma gondii as a source of cells to facilitate further investigation into the CD8 T cell mediated response against this pathogen. The TN T cells bound Ld -Gra6 tetramer and proliferated upon unspecific and peptide-specific stimulation. The TCR beta sequence of the Gra6-specific TN CD8 T cells is identical in its V- and J-region to the TCR beta harboured by a hybridoma line generated in response to Gra6 peptide. Adoptively transferred Gra6 TN CD8 T cells proliferated upon Toxoplasma infection in vivo and exhibited an activated phenotype similar to host CD8 T cells specific to Gra6. The brain of Toxoplasma-infected mice carried Gra6 TN cells already at day 8 post-infection. Both Gra6 TN mice as well as adoptively transferred Gra6 TN cells were able to significantly reduce the parasite burden in the acute phase of Toxoplasma infection. Overall, the Gra6 TN mouse represents a functional tool to study the protective and immunodominant specific CD8 T cell response to Toxoplasma both in the acute as well as the chronic phase of infection. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.
[PubMed - as supplied by publisher]

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