Wednesday, February 10, 2016

In vitro Activity of Sorghum bicolor Extracts, 3-deoxyanthocyanidins, Against Toxoplasma gondii

 2016 Feb 5. pii: S0014-4894(16)30016-9. doi: 10.1016/j.exppara.2016.02.001. [Epub ahead of print]


We investigated dried red leaf extracts of S. bicolor for activity against Toxoplasma gondii tachyzoites. S. bicolor red leaf extracts were obtained by bioassay-guided fractionation using ethanol and ethyl acetate as solvents. Analysis of the crude and fractionated extracts from S. bicolor using electrospray ionization mass spectrometry (ESI-MS) showed that they contained significant amounts of apigeninidin, luteolinidin, 7-methoxyapigeninidin, 5-methoxyapigeninidin, 5-methoxyluteolinidin, 5-7-dimethoxyapigeninidin or 5-7-dimethoxyluteolinidin, based on mass per charge (m/z). When tested in vitro, the IC50s for inhibitory activity against T. gondii tachyzoites' growth of the ethanol and ethyl acetate extracts were 2.3- and 4-fold, respectively, lower than their cytotoxic IC50s in mammalian cells. Ethyl acetate extracts fractionated in chloroform-methanol and chloroform had IC50s against T. gondii that were 56.1- and 3-fold lower than their respective cytotoxic IC50s in mammalian cells. These antiparasitic activities were found to be consistent with those of the respective pure 3-deoxyanthocyanidin compounds identified to be contained in the fractions in significant amounts. Further, we observed that, the position and number of methoxy groups possessed by the 3-deoyanthocyanidins influenced their antiparasitic activity. Together, our findings indicate that S. bicolor red-leaf 3-deoxyanthocyanidins-rich extracts have potent in vitro inhibitory activity against the proliferative stage of T. gondii parasites.
Copyright © 2016. Published by Elsevier Inc.


3-deoxyanthocyanidins extracts; Sorghum bicolor; Toxoplasma gondii; antiparasitic compounds
[PubMed - as supplied by publisher]

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