Wednesday, August 19, 2015

Impact of Toxoplasma gondii on Dendritic Cell Subset Function in the Intestinal Mucosa

 2015 Aug 17. pii: 1501137. [Epub ahead of print]
 

Abstract

The function of mucosal dendritic cell (DC) subsets in immunity and inflammation is not well understood. In this study, we define four DC subsets present within the lamina propria and mesenteric lymph node compartments based on expression of CD103 and CD11b. Using IL-12p40 YFP (Yet40) reporter mice, we show that CD103+CD11b- mucosal DCs are primary in vivo sources of IL-12p40; we also identified CD103-CD11b- mucosal DCs as a novel population producing this cytokine. Infection was preferentially found in CD11b+ DCs that were negative for CD103. Lamina propria DCs containing parasites were negative for IL-12p40. Instead, production of the cytokine was strictly a property of noninfected cells. We also show that vitamin A metabolism, as measured by ALDH activity, was preferentially found in CD103+CD11b+ DC and was strongly downregulated in all mucosal DC subsets during infection. Finally, overall apoptosis of lamina propria DC subsets was increased during infection. Combined, these results highlight the ability of intestinal Toxoplasma infection to alter mucosal DC activity at both the whole population level and at the level of individual subsets.
Copyright © 2015 by The American Association of Immunologists, Inc.
PMID:
 
26283477
 
[PubMed - as supplied by publisher]

1 comment:

Dorsa Kord said...

Hello,

My name is Dorsa. I am an intern with Rare Genomics Institute and RareShare this summer. We are helping research information on rare disease and I am looking to interview experts/patients about different diseases to help patients with these diseases.

I am working on disease Congenital Toxoplasmosis. It seems like you are an expert in this field and if it is so, we would really appreciate it if you could share your expertise with us through a phone interview.
The interview should take no longer than 30 minutes and we will send you the interview questions beforehand.
My email address is dorsa.rare@yahoo.com
Please contact me if you are interested and we can schedule the interview.

Best,
Dorsa