Wednesday, January 14, 2015

Sulfamethoxazole-trimethoprim associated with resveratrol for the treatment of toxoplasmosis in mice

2015 Jan 6;79C:17-23. doi: 10.1016/j.micpath.2015.01.001. [Epub ahead of print]
 
 
This study aimed to investigate the influence of sulfamethoxazole-trimethoprim (ST) associated with resveratrol on the enzymatic activities of acetylcholinesterase (AChE), adenylate kinase (AK), pyruvate kinase (PK), and creatine kinase (CK) in the brain of mice experimentally infected by Toxoplasma gondii. For that, 60 mice were divided into ten groups with 6 animals each: groups A to D composed by healthy mice and groups E to J consisting of animals infected by T. gondii (VEG strain). Animals started treatment 20 days post-infection for 10 consecutive days with oral doses of 0.5 mg kg-1 of ST (groups B and F), 100 mg kg-1 of free resveratrol (groups C and G) and inclusion complex of resveratrol (nanoparticles containing resveratrol) (groups D and H), as well as with an association of both drugs (groups I and J). The results showed increased (P < 0.001) AChE activity on infected animals (groups E-J) when compared to not-infected (A) animals, and also uninfected animals treated with ST (group B) had increased AChE activity. AK activity decreased (P < 0.001) in the infected and untreated (group E), differently from the other groups that did not differ. PK activity did not differ between groups (P > 0.05). When comparing control groups (uninfected (A) and infected (E)), we verified a significant (P < 0.001) increase in CK activity in the brain, and it is noteworthy that the animals treated with resveratrol associated with ST (group I and J) had similar CK activity to those animals from the group A. Treatment with the combination of ST and resveratrol was able to reduce (P < 0.05) the number of parasitic cysts in the brain, thus reduced inflammatory infiltrates in the liver, and prevented the occurrence of hepatocytes lesions due to toxoplasmosis in mice. Based on these results, it is possible to conclude that increased AChE and CK activities after T. gondii infection did not change with the treatment of ST-resveratrol association. In addition, decreased AK activity caused by T. gondii infection was normalized by ST-resveratrol treatment. T. gondii infection and treatment does not affect PK activity in brain.
Copyright © 2015 Elsevier Ltd. All rights reserved.

KEYWORDS:

ACh; AChE; ATP; Polyphenol; T. gondii
PMID:
25572158
[PubMed - as supplied by publisher]

1 comment:

Miyushi Izumisa said...

Good sharing, you may already know that Resveratrol can improve your heart, your afteries, your weight and your skin. I would like share a good resveratrol supplement which is Vineatrol Mixed Resveratrol, it has six different molecules of Resveratrol for a supplement of unmatched potency. Just 100mg of Vineatrol Mixed Resveratrol offers you more benefits than 100 glasses of red wine and has 20 times the potency of ordinary trans-Resveratrol supplements. Read more at:
http://kidbuxblog.com/trigger-your-longevity-gene-with-resveratrol/