Wednesday, September 17, 2014

Sustained Translational Repression of Lactate Dehydrogenase 1 in Toxoplasma gondii Bradyzoites is Conferred by a Small Regulatory RNA Hairpin

 2014 Sep 15. doi: 10.1111/febs.13048. [Epub ahead of print]


In response to environmental stresses, Toxoplasma gondii induces a global translational repression which allows for the remodeling of its transcriptome. While some transcripts are preferentially translated, another subset is translationally repressed and maintained in bradyzoites. Although little is known of how transcripts are targeted for sustained translational repression, the targeting likely operates through an RNA-centric mechanism relying on the recognition of cis-acting elements. In this study, we sought to determine if the targeting of transcripts through recognizable cis-acting elements could be responsible for the transcript-specific sustained translational repression displayed by Toxoplasma bradyzoites. We examined the UTRs of a translationally repressed gene, lactate dehydrogenase 1, and found a 40 nucleotide regulatory element in its 5'UTR. This element specifically induces translational repression in otherwise constitutively expressed transcripts. Mutational studies revealed that the formation of a small 16 nucleotide regulatory RNA hairpin is essential for this activity. We suggest that this hairpin may act as the nucleation site for the binding of a yet to be identified trans-acting factor that allows for the transcript to be targeted for translational repression removal from the active translational pool. To our knowledge, this is the first report characterizing a specific cis-acting element contributing to post-transcriptional gene regulation in Toxoplasma and suggests the presence of a pathway by which the parasites can recognize, identify, and specifically target transcripts for sustained translational repression under stressful conditions. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.


Toxoplasma gondii ; 5'UTR; RNA hairpin; lactate dehydrogenase; translational repression
[PubMed - as supplied by publisher]

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