Nat Commun. 2014 Jun 17;5:4098. doi: 10.1038/ncomms5098.
Plasticity and redundancy among AMA-RON pairs ensure host cell entry of Toxoplasma parasites
Lamarque MH1, Roques M1, Kong-Hap M1, Tonkin ML2, Rugarabamu G3, Marq JB3, Penarete-Vargas DM1, Boulanger MJ2, Soldati-Favre D3, Lebrun M1.
Malaria and toxoplasmosis are infectious diseases caused by the apicomplexan parasites Plasmodium and Toxoplasma gondii, respectively. These parasites have developed an invasion mechanism involving the formation of a moving junction (MJ) that anchors the parasite to the host cell and forms a ring through which the parasite penetrates. The composition and the assembly of the MJ, and in particular the presence of protein AMA1 and its interaction with protein RON2 at the MJ, have been the subject of intense controversy. Here, using reverse genetics, we show that AMA1, a vaccine candidate, interacts with RON2 to maintain the MJ structural integrity in T. gondii and is subsequently required for parasite internalization. Moreover, we show that disruption of the AMA1 gene results in upregulation of AMA1 and RON2 homologues that cooperate to support residual invasion. Our study highlights a considerable complexity and molecular plasticity in the architecture of the MJ.
- [PubMed - in process]