Heparin-binding proteomes of Toxoplasma gondii and Plasmodium falciparum

Proteomics. 2014 May 28. doi: 10.1002/pmic.201400003. [Epub ahead of print]

A comparative study on the heparin-binding proteomes of Toxoplasma gondii and Plasmodium falciparum

Zhang Y¹, Jiang N, Jia B, Chang Z, Zhang Y, Wei X, Zhou J, Wang H, Zhao X, Yu S, Song M, Tu Z, Lu H, Yin J, Wahlgren M, Chen Q.

Abstract

Toxoplasma gondii is an obligatory intracellular apicomplexan parasite which exploits host cell surface components in cell invasion and intracellular parasitization. Sulfated glycans such as heparin and heparan sulfate have been reported to inhibit cell invasion by T. gondii and other apicimplexan parasites such as Plasmodium falciparum. The aim of this study was to investigate the heparin-binding proteome of T. gondii. The parasite-derived components were affinity-purified on the heparin moiety followed by mass-spectrometry finger-printing of the proteins. The heparin-binding proteins of T. gondii and P. falciparum were compared based on functionality and affinity to heparin. Among the proteins identified, the invasion-related parasite ligands derived from tachyzoite/merozoite surface and the secretory organelles were prominent. However, the profiles of the proteins were different in term of affinity to heparin. In T. gondii, the proteins with highest affinity to heparin were the intracellular components with functions of parasite development contrasted to that of P. falciparum, of which the rhoptry-derived proteins were prominently identified. The profiling of the heparin-binding proteins of the two apicomplexan parasites not only explained the mechanism of heparin-mediated host cell invasion inhibition, but also, to certain extents, revealed that the action of heparin on the parasite extended after endocytosis. This article is protected by copyright. All rights reserved.
This article is protected by copyright. All rights reserved.

KEYWORDS:

Plasmodium falciparum; Toxoplasma gondii; cell invasion; heparin; heparin-binding proteome

PMID:

24888565

[PubMed - as supplied by publisher]