Exp Parasitol. 2012 Nov 30. pii: S0014-4894(12)00357-8. doi: 10.1016/j.exppara.2012.11.019. [Epub ahead of print]
Induction of sulfadiazine resistance in vitro in Toxoplasma gondii
Doliwa C, Escotte-Binet S, Aubert D, Velard F, Schmid A, Geers R, Villena I.
Laboratoire de Parasitologie-Mycologie, EA 3800, SFR CAP-Santé FED 4231, Université de Reims Champagne-Ardenne, 51 rue Cognacq-Jay, 51095 Reims Cedex, France. Electronic address: email@example.com.
We induced sulfadiazine resistance in two sulfadiazine sensitive strains of T. gondii, RH (Type I) and ME-49 (Type II) in vitro by using drug pressure. At first, sulfadiazine susceptibility of the two sensitive strains and two naturally resistant strains of T. gondii was evaluated on Vero cells using an enzyme-linked immunosorbent assay (ELISA). The IC(50) values of sulfadiazine were 77 μg/mL for RH, 51 μg/mL for ME-49 and higher than 1,000 μg/mL for the two natural resistant strains. Secondly, induced resistance of the strains by gradually increase sulfadiazine concentration was verified by this test, which resulted IC(50) values at higher than 1,000 μg/mL. In conclusion we developed in vitro two sulfadiazine resistant strains called RH-R(SDZ) and ME-49-R(SDZ). These strains resistant to sulfadiazine would be useful to characterize resistance mechanisms to sulfadiazine.
PMID: 23206954 [PubMed - as supplied by publisher]