Wednesday, May 02, 2012

Lysine acetylation is widespread on proteins of diverse function and localization in the protozoan parasite Toxoplasma gondii

Eukaryot Cell. 2012 Apr 27. [Epub ahead of print]

Lysine acetylation is widespread on proteins of diverse function and localization in the protozoan parasite Toxoplasma gondii

Jeffers V, Sullivan WJ Jr.

Department of Pharmacology and Toxicology.  Indiana University School of Medicine.

While histone proteins are the founding members of lysine acetylation substrates, it is now clear that hundreds of other proteins can be acetylated in multiple compartments of the cell. Our knowledge of the scope of this modification throughout the kingdom of life is beginning to emerge as proteome-wide lysine acetylation has been documented in prokaryotes, Arabidopsis thaliana, Drosophila melanogaster, and human cells. Using LC-MS/MS to identify parasite peptides enriched by immunopurification with acetyl-lysine antibody, we produced the first proteome-wide analysis of acetylation for a protozoan organism, the opportunistic apicomplexan parasite Toxoplasma gondii. The results show that lysine acetylation is abundant in the actively proliferating tachyzoite form of the parasite, which causes acute toxoplasmosis. Our approach successfully identified known acetylation marks on Toxoplasma histones and α-tubulin, and detected over 400 novel acetylation sites on a wide variety of additional proteins, including those with roles in transcription, translation, metabolism, and stress responses. Importantly, an extensive set of parasite-specific proteins are acetylated in the parasite, including those found in organelles unique to Apicomplexa. Our data provide a wealth of new information that improves our understanding of the evolution of this vital regulatory modification while potentially revealing novel therapeutic avenues. We conclude from this study that lysine acetylation was prevalent in the early stages of eukaryotic cell evolution and occurs on proteins involved in a remarkably diverse array of cellular functions, including those that are specific to parasites.

PMID: 22544907 [PubMed - as supplied by publisher]

1 comment:

Tank said...

Congratulations!