Thursday, February 09, 2012

Animal Models for Toxoplasma gondii Infection

Curr Protoc Immunol. 2012 Feb;Chapter 19:Unit19.3.

Animal Models for Toxoplasma gondii Infection.

Subauste C.

Case Western Reserve University School of Medicine, Cleveland, Ohio.

Toxoplasma gondii is a protozoan of worldwide distribution. This unit describes murine models of acute T. gondii infection, toxoplasmic encephalitis, and Toxoplasma retinochoroiditis. T. gondii infection in SCID mice allows the study of T cell-independent mechanisms of defense. The uracil auxotroph strain cps1-1 and temperature-sensitive mutant strains of T. gondii allow studies of immunization and adoptive transfer. In vivo study of parasite host-interaction is possible with the use of parasites that express fluorescent proteins and model antigens, plus the use of transgenic mice that express the appropriate T cell receptor and fluorescently labeled leukocytes. Parasites that express bioluminescent markers make it possible to study the dynamics of infection in real time using bioluminescence imaging. Support protocols present methodology for evaluation of progression of infection and immune response to the parasite, the maintenance of T. gondii tissue cysts and tachyzoites, as well as preparation of T. gondii lysate antigens. Curr. Protoc. Immunol. 96:19.3.1-19.3.23. © 2012 by John Wiley & Sons, Inc.

PMID: 22314833 [PubMed - in process]

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