Saturday, October 15, 2011

Evolutionarily divergent, unstable filamentous actin is essential for gliding motility in apicomplexan parasites

PLoS Pathog. 2011 Oct;7(10):e1002280. Epub 2011 Oct 6

Evolutionarily divergent, unstable filamentous actin is essential for gliding motility in apicomplexan parasites

Skillman KM, Diraviyam K, Khan A, Tang K, Sept D, Sibley LD

SourceDepartment of Molecular Microbiology, Washington University School of Medicine, St. Louis, Missouri.

Abstract
Apicomplexan parasites rely on a novel form of actin-based motility called gliding, which depends on parasite actin polymerization, to migrate through their hosts and invade cells. However, parasite actins are divergent both in sequence and function and only form short, unstable filaments in contrast to the stability of conventional actin filaments. The molecular basis for parasite actin filament instability and its relationship to gliding motility remain unresolved. We demonstrate that recombinant Toxoplasma (TgACTI) and Plasmodium (PfACTI and PfACTII) actins polymerized into very short filaments in vitro but were induced to form long, stable filaments by addition of equimolar levels of phalloidin. Parasite actins contain a conserved phalloidin-binding site as determined by molecular modeling and computational docking, yet vary in several residues that are predicted to impact filament stability. In particular, two residues were identified that form intermolecular contacts between different protomers in conventional actin filaments and these residues showed non-conservative differences in apicomplexan parasites. Substitution of divergent residues found in TgACTI with those from mammalian actin resulted in formation of longer, more stable filaments in vitro. Expression of these stabilized actins in T. gondii increased sensitivity to the actin-stabilizing compound jasplakinolide and disrupted normal gliding motility in the absence of treatment. These results identify the molecular basis for short, dynamic filaments in apicomplexan parasites and demonstrate that inherent instability of parasite actin filaments is a critical adaptation for gliding motility.

PMID:21998582[PubMed - in process]

2 comments:

the red silver fox said...

I leave a comment only because as I read through your words, it sounded like the dance they write for the show, "Sanctuary".

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