Thursday, June 16, 2011

Mol Biochem Parasitol. 2011 Jun 2. [Epub ahead of print]

A transmembrane domain containing pellicle protein of Toxoplasma gondii enhances virulence and invasion after extracellular stress

Payne TM, Lund PJ, Knoll LJ

SourceDepartment of Medical Microbiology and Immunology, University of Wisconsin School of Medicine and Public Health, Microbial Sciences Building Room 3345, 1550 Linden Drive, Madison, WI 53706, United States.

To identify Toxoplasma gondii genes important in the establishment of a persistent infection, we previously used signature-tagged mutagenesis to identify mutants with reduced cyst numbers in the brains of mice. One of the mutants, 95C5, has an insertion within a predicted six transmembrane domain protein, which localizes to the parasite pellicle, thus we named it transmembrane pellicle protein 1 (TgTPP1). Although the 95C5 mutant was found be reduced in its ability to form brain cysts, it is defective during acute infection. Addition of TgTPP1 expressed from its endogenous promoter restored the acute lethality of the 95C5 mutant to parental levels. The 95C5 mutant does not have a growth defect in standard tissue culture conditions; however, we found a significant defect in host cell penetration after extracellular stress. Overall, TgTPP1 may function during acute infection by enhancing the parasites ability to invade after extracellular stress.

Copyright © 2011 Elsevier B.V. All rights reserved.

PMID:21669237[PubMed - as supplied by publisher]

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