Wednesday, July 28, 2010

Direct evidence of O-GlcNAcylation in the apicomplexan Toxoplasma gondii: a biochemical and bioinformatic study

Amino Acids. 2010 Jul 27. [Epub ahead of print]

Direct evidence of O-GlcNAcylation in the apicomplexan Toxoplasma gondii: a biochemical and bioinformatic study

Perez-Cervera Y, Harichaux G, Schmidt J, Debierre-Grockiego F, Dehennaut V, Bieker U, Meurice E, Lefebvre T, T Schwarz R.

Unit of Structural and Functional Glycobiology, CNRS-UMR 8576, IFR 147, Université de Lille 1, Cité Scientifique, 59655, Villeneuve d'Ascq, France.

Abstract
Toxoplasma gondii and Plasmodium falciparum are apicomplexan parasites responsible for serious diseases in humans. Many studies have focused on the post-translational modifications (PTMs) found in the two protists including phosphorylation, acetylation or SUMOylation but only a few of these are concerned with the nuclear and cytosolic-specific glycosylation O-GlcNAcylation. O-GlcNAcylation is a highly dynamic PTM-regulated by the ON and OFF enzymes: O-GlcNAc transferase and O-GlcNAcase-that can compete with phosphorylation but its function remains unclear. In this work, we directly prove the O-GlcNAcylation in T. gondii using antibodies specifically directed against the modification and we strongly suggest its occurrence in P. falciparum. We found that the inducible 70 kDa-Heat Shock Protein is O-GlcNAcylated, or associated with an O-GlcNAc-partner, in T. gondii. Using anti-OGT antibodies we were able to detect the expression of the glycosyltransferase in T. gondii cultured both in human foreskin fibroblast and in Vero cells and report its putative sequence. For the first time the presence of O-GlcNAcylation is unequivocally shown in T. gondii and suspected in P. falciparum. Since the O-GlcNAcylation is implicated in many biological fundamental processes this study opens a new research track in the knowledge of apicomplexans' life cycle and pathogenic potential.

PMID: 20661758 [PubMed - as supplied by publisher]

3 comments:

劉士賢 said...

做好事,不需要給人知道,雖然只是一件微不足道的事,但我相信,這會帶給我快樂。..................................................

施以王雅玲音 said...

死亡是悲哀的,但活得不快樂更悲哀。............................................................

秋珍雅昀 said...

人們不缺少力量,他們缺少意志。..................................................