Sunday, May 23, 2010

A NEW ATYPICAL HIGHLY MOUSE VIRULENT TOXOPLASMA GONDII GENOTYPE ISOLATED FROM A WILD BLACK BEAR IN ALASKA

J Parasitol. 2010 Apr 5:1. [Epub ahead of print]

A NEW ATYPICAL HIGHLY MOUSE VIRULENT TOXOPLASMA GONDII GENOTYPE ISOLATED FROM A WILD BLACK BEAR IN ALASKA

Dubey JP, Chellaiah R, Ferreira L, Kwok O, Sinnett D, Majumdar D.

Abstract
Most strains of Toxoplasma gondii isolated in North America and Europe are grouped into three (Types I, II, III) genetic types and are considered clonal. Recent evidence suggests that illness due to toxoplasmosis in immunocompetent persons may be related to infection with atypical genotype; these strains are mouse virulent. In the present study, a new mouse virulent atypical T. gondii genotype was isolated from an asymptomatic black bear (Ursus americanus) from Alaska, USA. The bear had a titer of 1:1,600 in the modified agglutination test for T. gondii. Swiss Webster out-bred mice inoculated with bear heart homogenate died of acute toxoplasmosis, 12 days p.i. Cats fed tissues from chronically infected animals (30 day infection) shed oocysts but cats fed acutely infected mice ( 12 and 18 days p.i.) did not. The isolate (designated TgBbUS1) was mouse virulent, mice inoculated with 1 oocyst died of acute toxoplasmosis. The restricted fragment length polymorphism using 10 markers revealed that the strain possessed an atypical genotype; type I allele at loci SAG1, (5'-3')SAG2, SAG3, c22-8, c29-2, L358, and Apico, type II allele at locus alt.SAG2, and type III allele at loci BTUB, GRA6, and PK1. DNA sequencing at intron loci EF1, HP2 and UPRT1 revealed that the TgBbUS1 is a divergent T. gondii strain. These results indicate that mouse virulent atypical T. gondii genotypes are also circulating in wildlife in the US.

PMID: 20486739 [PubMed - as supplied by publisher]

1 comment:

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