Nat Methods. 2010 Mar 7. [Epub ahead of print]
Toxoplasma secreting Cre recombinase for analysis of host-parasite interactions
Koshy AA, Fouts AE, Lodoen MB, Alkan O, Blau HM, Boothroyd JC.
 Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, California, USA.  Division of Infectious Disease, Department of Internal Medicine, Stanford University School of Medicine, Stanford, California, USA.
We describe a Toxoplasma gondii strain that will permit the use of site-specific recombination to study the host-parasite interactions of this organism. This Toxoplasma strain efficiently injects a Cre fusion protein into host cells. In a Cre-reporter cell line, a single parasite invasion induced Cre-mediated recombination in 95% of infected host cells. By infecting Cre-reporter mice with these parasites, we also monitored host-cell infection in vivo.
PMID: 20208532 [PubMed - as supplied by publisher]