Immunity. 2009 Aug 21;31(2):342-55
Dynamics of T cell, antigen-presenting cell, and pathogen interactions during recall responses in the lymph node
Chtanova T, Han SJ, Schaeffer M, van Dooren GG, Herzmark P, Striepen B, Robey EA.
Department of Molecular and Cell Biology, Life Sciences Addition, University of California, Berkeley, CA 94720, USA.
Memory T cells circulate through lymph nodes where they are poised to respond rapidly upon re-exposure to a pathogen; however, the dynamics of memory T cell, antigen-presenting cell, and pathogen interactions during recall responses are largely unknown. We used a mouse model of infection with the intracellular protozoan parasite, Toxoplasma gondii, in conjunction with two-photon microscopy, to address this question. After challenge, memory T cells migrated more rapidly than naive T cells, relocalized toward the subcapsular sinus (SCS) near invaded macrophages, and engaged in prolonged interactions with infected cells. Parasite invasion of T cells occurred by direct transfer of the parasite from the target cell into the T cell and corresponded to an antigen-specific increase in the rate of T cell invasion. Our results provide insight into cellular interactions during recall responses and suggest a mechanism of pathogen subversion of the immune response.
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
PMID: 19699173 [PubMed - in process]