Genome Biol. 2008 Jul 21;9(7):R116. [Epub ahead of print]
The proteome of Toxoplasma gondii: integration with the genome provides novel insights into gene expression and annotation
Xia D, Sanderson SJ, Jones AR, Prieto JH, Yates JR 3rd, Bromley E, Tomley FM, Lal K, Sinden RE, Brunk BP, Roos DS, Wastling JM.
ABSTRACT: BACKGROUND: Although the genomes of many of the most important human and animal pathogens have now been sequenced, our understanding of the actual proteins expressed by these genomes and how well they predict protein sequence and expression is still deficient. We have used three complementary approaches (two-dimensional electrophoresis, gel LC-MS/MS and MudPIT) to analyse the proteome of Toxoplasma gondii, a parasite of medical and veterinary significance and have developed a public repository for these data within ToxoDB, making for the first time proteomics data an integral part of this key genome resource. RESULTS: The draft genome for Toxoplasma predicts around 8000 genes with varying degrees of confidence. Our data demonstrate how proteomics can inform these predictions and help discover new genes. We have identified nearly one third (2252) of all the predicted proteins, with 2477 intron-spanning peptides providing supporting evidence for correct splice site annotation. Functional predictions for each protein and key pathways were determined from the proteome. Importantly, we show evidence for many proteins that match alternative gene models, or previously unpredicted genes. For example, approximately 15% of peptides matched more convincingly to alternative gene models. We also compared our data with existing transcriptional data in which we highlight apparent discrepancies between gene transcription and protein expression. CONCLUSIONS: Our data demonstrate the importance of protein data in expression profiling experiments and highlight the necessity of integrating proteomic with genomic data so that iterative refinements of both annotation and expression models are possible.
PMID: 18644147 [PubMed - as supplied by publisher]