Parasite Immunol. 2008 Mar 3 [Epub ahead of print]
Protective effect of a multiantigenic DNA vaccine against Toxoplasma gondii with co-delivery of IL-12 in mice
Cui YL, He SY, Xue MF, Zhang J, Wang HX, Yao Y.
Department of Parasitology, School of Medicine, Shandong University, Shandong, China.
In this study, we constructed a multiantigenic DNA vaccine, pSAG1-ROP2-SAG2 and examined its effect with co-delivery of a plasmid encoding IL-12 (pIL-12) as an adjuvant in BALB/c mice against Toxoplasma gondii. After a lethal challenge of T. gondii RH strain, survival of the mice immunized with this pSAG1-ROP2-SAG2 vaccine was significantly prolonged in comparison to the control groups. Furthermore, the protection was significantly augmented by pIL-12 co-delivery. As demonstrated by lymphocyte proliferation assay, cytokine and antibody level determinations, the humoral and Th1-type cellular responses elicited by this multiantigenic DNA vaccine were significantly stronger than those elicited by double-antigenic, or single-antigenic DNA vaccines. Our data suggest that multiantigenic DNA vaccine with pIL-12 co-delivery is a very effective approach in the protection against T. gondii.
PMID: 18331395 [PubMed - as supplied by publisher]