Eukaryot Cell. 2008 Jan 4 [Epub ahead of print]
Toxoplasma gondii and Cryptosporidium parvum lack detectable DNA cytosine methylation.
Gissot M, Choi SW, Thompson RF, Greally JM, Kim K.
Departments of Medicine (Infectious Diseases) and Microbiology & Immunology, Albert Einstein College of Medicine, Bronx, NY 10461, USA; Jean Mayer USDA Human Nutrition Center on Aging at Tufts University, 711 Washington Street, Boston, MA 02111, USA.; Departments of Medicine (Hematology) and Molecular Genetics, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
Epigenetic factors play a role in expression of virulence traits in Apicomplexa. Apicomplexan genomes encode putative DNA cytosine methylation enzymes. To assess the presence of cytosine methylation of T.gondii and C. parvum DNA, we used mass spectrometry analysis and confirmed that these organisms lack detectable methylcytosine in their DNA.
PMID: 18178772 [PubMed - as supplied by publisher]