Tuesday, November 13, 2007

A transient forward targeting element for microneme regulated secretion in Toxo

Biol Cell. 2007 Nov 12; [Epub ahead of print]

A transient forward targeting element for microneme regulated secretion in Toxoplasma gondii

Brydges SD, Harper JM, Parussini F, Coppens I, Carruthers VB

Background information. Accurate sorting of proteins to the three types of secretory granules in Toxoplasma gondii is crucial for successful cell invasion by this obligate intracellular parasite. As in other eukaryotic systems, propeptide sequences are a common yet poorly understood feature of proteins destined for regulated secretion, which for Toxoplasma occurs through two distinct invasion organelles, rhoptries and micronemes. Microneme discharged during parasite apical attachment plays a pivotal role in cell invasion by delivering adhesive proteins for host receptor engagement. Results. We show here that the small micronemal proprotein MIC5 undergoes proteolytic maturation at a site beyond the Golgi and only the processed form of MIC5 is secreted via the micronemes. Proper cleavage of the MIC5 propeptide relies on an arginine residue in the P1' position, though P1' mutants are still cleaved to a lesser extent at an alternative site downstream of the primary site. Nonetheless, this aberrantly cleaved species still correctly traffics to the micronemes, indicating that correct cleavage is not necessary for micronemal targeting. In contrast, a deletion mutant lacking the propeptide was retained within the secretory system, principally in the endoplasmic reticulum. The MIC5 propeptide also supported correct trafficking when exchanged for the M2AP propeptide, which was recently shown to also be required for micronemal trafficking of the TgMIC2-M2AP complex (Harper et al., Mol Biol Cell (2006) 4551-63). Conclusion. Our results illuminate common and unique features of micronemal propeptides in their role as trafficking facilitators.

PMID: 17995454 [PubMed - as supplied by publisher]

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